《植物生理学报》 2012, 48(4): 359-363

小叶龙竹(Dendrocalamus barbatus)愈伤组织诱导及植株再生

王娟¹, 毕玮¹, 普晓兰¹, 陈芳², 彭桂莎¹, 杨宇明^2,*

1西南林业大学, 国家林业局西南生物多样性保育重点实验室, 昆明650224; 2云南省林业科学院, 昆明650201

通信作者：杨宇明；E-mail: yymbamb@163.com；Tel: 0871-5150250

摘　要：

以小叶龙竹种子为外植体, 通过研究MS培养基中不同植物生长调节剂浓度组合对外植体愈伤组织诱导和不定芽分化的影响以及不同配比对生根的作用, 建立了稳定的繁殖再生体系。试验结果表明愈伤组织诱导的最适培养基为MS+2,4-D5.0 mg·L-1; 不定芽分化的最适培养基为MS+2,4-D 0.5 mg·L-1+6-BA 1.0 mg·L-1+KT 0.25 mg·L-1; 小苗的最适生根培养基为1/2MS+6-BA 0.5 mg·L-1+NAA 1.0 mg·L-1+IBA 0.4 mg·L-1。建立的繁殖再生体系将为进一步利用分子生物学技术对竹类植物进行遗传改良奠定基础。

关键词：小叶龙竹; 愈伤组织诱导; 植株再生

收稿：2012-01-30   修定：2012-02-28

资助：国家林业局948技术引进项目(2008-4-30)、云南省自然科学基金面上项目(2009CD073)和云南省中青年学术技术带头人后备人才培养项目(2010CI016)。

Callus Induction and Plantlet Regeneration of Dendrocalamus barbatus Hsueh et D. Z. Li

WANG Juan¹,BI Wei¹, PU Xiao-Lan¹,CHEN Fang², PENG Gui-Sha¹,YANG Yu-Ming^2,*

¹State Forestry Administration Key Lab of Biodiversity Conservation in Southwest Region of China, Southwest Forest University, Kunming 650224, China; ²Yunnan Academy of Forestry, Kunming 650201, China

Corresponding author: YANG Yu-Ming; E-mail: yymbamb@163.com; Tel: 0871-5150250

Abstract:

In this study, calli were induced from seed explants of Dendrocalamus barbatus. According to the research of the effects of different kinds and concentrations of plant growth regulator combination in MS medium on callus induction and bud differentiation and the influence of different regulator on rooting effect, a stable regeneration system was set up. The result indicated that the best medium for callus induction contained MS basal medium supplemented with 2,4-D 5.0 mg·L^-1; the feasible medium for adventitious shoots differentiation from callus was MS+2,4-D 0.5 mg·L^-1+6-BA 1.0 mg·L^-1+KT 0.25 mg·L^-1; and the best medium for seedling rooting was 1/2MS+6-BA 0.5 mg·L^-1+NAA 1.0 mg·L^-1+IBA 0.4 mg·L^-1. The establishment of regeneration system lays a foundation for the further use of molecular biology techniques for the genetic improvement of bamboo plants.

Key words: Dendrocalamus barbatus; callus induction; plant regeneration